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Isozyme analysis was conducted for cultivar identification of l44 persimmon (Diospyros kaki Thunb.) cultivars that consisted of 112 Korean natives and 32 Japanese cultivars. Electrophoretic analysis of isozymes revealed polymorphism in the tour isozyme systems including glucosephosphate isomerase (GPI), malate dehydrogenase (MDH), peroxidase (PER), and phosphoglucomutase (PGM). Twenty-one different band patterns (A to U) of GPI were identified and Korean native had mostly C, F and J zymotypes. In MDH, tour different zymotypes (A to D) were revealed, among which A and B zymotypes were most frequent while the D type frequently appeared in cultivars collected in Gangwon province. On the basis of isozyme analysis, cultivars could be grouped into 92 categories; and it also be able to fingerprint 64 different cultivars. ´Okcheon-Susi and Okcheon-Bansi´, ´Okcheon-Weolhasi and Okcheon-Gaeweolhasi´, ´Gyeongsan-Bansi, Gyeongsan-Namyangsusi and Changryeong-Bansi´, ´Kwangju-Pasi and Mokpo-Pasi´, ´Jinyang-Mulbansi and Hiratanenashi´, and ´Kawabata and Ro-19´ cultivars were identified to similar cultivar by multivariate and isozyme analyses, respectively.
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